Koch Lab:Inventory/Unlabeled tubulin

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lot details

Refer to: http://openwetware.org/index.php?title=Koch_Lab:Protocols/Kinesin/Tubulin_resuspension_and_polymerization&oldid=307098#Non-labeled_tubulin_resuspension


  • Use cold GPEM, add 180 ul of GPEM to 1mg lyophilized tubulin
  • Add 20 ul of cold cushion buffer
  • Mix, then make 5 ul aliquots in 500 ul tubes
    • Tubes will be put into 50 mL centrifuge tubes, labeled "tubulin 6/15/09, 5 λ aliquots"

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