Knight:Protein DNA binding/Option 1/quick reference recipe

To make 1mL ...

• 100μL 50% glycerol
• 50μL 1M potassium glutamate
• 30μL 500mM HEPES-NaOH (pH7.9)
• 10μL 10% NP-40
• 10μL 5M potassium acetate
• 12.5μL 4M potassium chloride
• 5μL 1M magnesium chloride
• 1μL 20mM zinc sulfate
• 2μL 1mg/mL BSA
• 779.5μL H₂O

To make 1000mL ...

• 100mL 50% glycerol
• 50mL 1M potassium glutamate
• 30mL 500mM HEPES-NaOH (pH7.9)
• 10mL 10% NP-40
• 10mL 5M potassium acetate
• 12.5mL 4M potassium chloride
• 5mL 1M magnesium chloride
• 1mL 20mM zinc sulfate
• 200μL 10mg/mL BSA
• 781.3mL H₂O