Knight:Protein DNA binding/Option 1/quick reference recipe
From OpenWetWare
Jump to navigationJump to search
To make 1mL ...
- 100μL 50% glycerol
- 50μL 1M potassium glutamate
- 30μL 500mM HEPES-NaOH (pH7.9)
- 10μL 10% NP-40
- 10μL 5M potassium acetate
- 12.5μL 4M potassium chloride
- 5μL 1M magnesium chloride
- 1μL 20mM zinc sulfate
- 2μL 1mg/mL BSA
- 779.5μL H2O
To make 1000mL ...
- 100mL 50% glycerol
- 50mL 1M potassium glutamate
- 30mL 500mM HEPES-NaOH (pH7.9)
- 10mL 10% NP-40
- 10mL 5M potassium acetate
- 12.5mL 4M potassium chloride
- 5mL 1M magnesium chloride
- 1mL 20mM zinc sulfate
- 200μL 10mg/mL BSA
- 781.3mL H2O
