Knight:Part construction plans
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Part designs and construction plans -- Knight Lab
- Ongoing: library of transcriptional terminators, characterization of terminators. Cassie Huang
- Ongoing: library of zinc finger transcriptional repressors, characterization, design, construction: Reshma Shetty
- Ongoing: new low copy number vectors for E. coli in standard component form; compatible set of antibiotic resistance parts: Reshma Shetty
- Ongoing: library of RNA splicing constructs for dynamic post transcriptional modification of mRNA species: Austin Che
- Ongoing: library of yeast artificial chromosome components: Tom Knight
- Ongoing: library of transposon and transposome components: Tom Knight
- Ongoing: scent producing genes in E. coli, substrate production and optimization of the metabolic pathways: MIT IGEM team
- Ongoing: Optimization of standard assembly process with robotic implementation: Tom Knight, Randy Rettberg, Meagan Lizzaro
- standardized production techniques for chemically competent TOP10 cells
- standardized production techniques for backbone plasmids
- protocol design for three antibiotic assembly, manual and robotic
- Ongoing: parts for construction of 2-D protein crystals, based on S-layer proteins: Julie Norville
- Planned: library of all open reading frames in the Mesoplasma florum genome: Tom Knight
- Planned: Editing constructs for yeast artificial chromosomes: Tom Knight
- Planned: Yeast selection marker set: Tom Knight
- Planned: Protein fusion tag library (Flag, Streptactin, 6-His (both ends), protein cleavage sites, degradatation tags: Tom Knight / Julie Norville
- Interest: Library of promoters with varying strengths.
- Interest: Better characterization based on turbidistat control of cell state and online induction control and measurement