Knight:Loading dye

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6X loading dye to mix with DNA samples for use in agarose gel electrophoresis.


  • Ficoll 400
  • Deionized water
  • Orange G dye


Dissolve 1.5 g of Ficoll in 10 mL of deionized water. Add very small amounts of Orange G dye such that the loading dye is dark orange. Store in small aliquots at 4°C (room temperature is okay too). To use, add and mix 1/5th volume of loading dye to DNA solutions prior to loading into the wells of gels.