Klapperich Lab:Pressing Zeonor Slides for DNA Growth
Press Zeonor pellet into 1mm slide.
- Carver Hot Press
- Mass Scale
- Zeonor 750 pellets
- 0.8mm shims
- Mass out 5900 mg Zeonor pellets
- Spread in uniform circle on Hot Press slide
- Apply three orange 0.8mm shims in this configuration:
- Press at 2000 psi for 5-6minutes
- List troubleshooting tips here.
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Relevant papers and books
- Goldbeter A and Koshland DE Jr. An amplified sensitivity arising from covalent modification in biological systems. Proc Natl Acad Sci U S A. 1981 Nov;78(11):6840-4.
- JACOB F and MONOD J. Genetic regulatory mechanisms in the synthesis of proteins. J Mol Biol. 1961 Jun;3:318-56.
- Mark Ptashne. A genetic switch. Cold Spring Harbor, N.Y.: Cold Spring Harbor Laboratory Press, 2004. ISBN:0879697164
- Who has experience with this protocol?