Khosla:pCK-KPY144

pCK-KPY144;

TAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAATTCCCCTGTAGAAATAATTTTGTTTAACTTTAATAAGGAGATATACCATGGGTAGGGGAA
TCGACCTGGACAGCTTCGCCACCACCCTGCACGCCGACGACCCGGTGGTGCGCGACCACCGCGTGCACGGCGTGCGGATCCTGCCCGGCGTGGTGTATCT
CGACATGATTCTGCGCGCGGCGATGGCGGCCGGTTATCAGCCCGGCGACCTGACCATCCGGGAGGCGCTGTTCCAGCGTCCGGTCGCCCTCGCCGACGAC
GGCGCCGTGCGCATCGCGTTCCGGGTGGATCGTCCCACCGGGCGGATCGCCATCGACGTGCAGCGACTCGACCACGCGGGCGCGCCGTACGGGCCGGTCG
ATTCCGTGGCGGAATGTGTTGTGGTGCGGTCGAGTTCGCCGACCGAGCCACCGGCATCGGATACCGCGGTCGGTTCGGCGCAGGCGCGCGGCATGGCAGA
GATGTACGCGCAGAGTGGTAGCCGTGGCATCGAGCATCGCGATTTCATGCGCGCCACCGGTCATTTCGCGCGCAGCGGCGACATCGTCACCGCGACGATG
GGGCTCGGCCCCGAGGCAGCCGCGCACCTGCCCGGGTTTCTGCTGCATCCCACGTTCCTGGACGCCTCGACGATCGTCCCGTTCGTCGATCCACAACGGT
TGGGTGCCGACGACGACAGCGCGTTCATCCCGATCTACTTCGGTGACGTGCACGCCTGGCAGGCAACGCCCGAACAGGTCTTCGTCAGGGCGTCGGTACG
CGGCGGCGGGCACGGCGGCGACCTGCTCGAAGCCGACGTGGCCCTCTACGCCCCGGACGGATCGCCGGTGGTGCGCTATCGGCTGACCTCCAAGCGTGTG
CGCGGCAGCGCGGACATCCACCGGTTGCTGACGCCGACGGAGGCGCCCGCGCCGCAACAGCCCGTACCTGCCAGCTCAACGGTCATGCAATACGATCCGG
CGGCTACCGGACCGGTATCCGCCGCGCAGTATGGTCCGCCGGCCGCCAGCCCGGCGCAGGGCCCGTCGACGAAGGACCCACGCGCACTGATCACCGCCGA
CCTGGCTGCTCTGGTCGCGGCCGAATTGGGTGCCGATGCTGCCGGTATCGCCACCGACGTGGGGTTCTATGAACTCGGCTTGGATTCCGGCCGACTGCTG
CGGCTGACCGCCGAACTGGAAGGACGGGTCGGCGAGCCGCTGTATCCGACACTGCTGTTCGAGTACCAGACCATCGACGCGCTGGCCGGTCACCTCGCCG
AGACAGTCGGCGCGAACTATCGGCCCGACGCCGCCACGACCGCGCACCAACCCGGCGCAGGTACGACACCGGCTGAATTCGCCACCCAGTCAACGGGACT
CAGTCGGGCACCGCAGGTGGCAGCCCCACCGAGCGGATCAACCGACCCCGGTCCCGCGCCACACGCAGCCACACCGCCGGGATCAAACGCACTGGGATCG
ACTCCGCAGGCGGTTACCCCGGCGAGCGGATCGACCGACCTTGGTCCTGCTCCGTATGCGGCTACACCGACGACAGGATCAACCGGACAGGGTCCGGCGC
CGCAGGCGGTTACCCTGACGACCGGATCGACCGACCCCGCCCCCGCGCCGCAAGCGGCAGTCCCCACGACACCATCGACTGGTTCGGTACCGCAAGCAGC
CGCCCCGAAAGCAGGCTCGACCGAATTCCGTTCGGCGCAATCGGACGCGCCGATTGCGGATCCGCCCTTCGCATCGTCGACAACTGTCGCCCCCGCGTCA
GCGGCCAGGGACGAGCTGGTCACCTTCGGTTTCGACTGGGAAACCGACGCCACCGCACATCCTCGCCGGGAAGCGATCGGTCTGCTCGTCTTCGACACGG
CCGACCACCCCTACCGTCACGCAGGGGCGGCCGCCGTGGTCAGGGTGCGGCCTGGTGATGAGTTTGTGTCGGGCGCAGTGGATTTCGCTGTCAACCCGAC
AGATCCGCGGCATATGGGTCTGCTGGTCGAGCAGCTGACGGCTCGCGGGCTGCGCTTCGATGCCGTGTTGTATCTGTGGCCCGAATCGATCGGCGACGAT
GACTCGCGGGACTTGATCGAGCGGGTGTACTTGCCGCTGCGTGATCTCGTGCGTGCGACAGCCGGGCTATCCGAATCGCCGGTGCGGCTGCTGCTCGCTG
TCACACGCGCCACACCCGCGGGTGAGCTGAGTTGCGCTCTTCTGGGTGGGTTCGCGCGAACGCTGCGGCTGGAGCAGCCGCAGCCGATCCTGCGCGCCGT
GCAGTGCGATCCGGGTGCGGCGATCGATGACATCGTGGCCGCCGAACTGGGCGTGGAGGAACGTCACCCGGCCACCGAGATCCGCTATCGCGGTGGCCGA
CGCGAGATCAGGTCGCCGCGCCTAGATACGGATTCCGCGGGCGCCACCGGGGACCCGGTTCTGCGGAGGGGATCGGTGGTCGTGGTGACCGGCGGGTTCG
GCGGCATCGCCCGGCATCTGGCGCGCCGACTCGCGACCGACTACAGCGCGCGTCTGGTGCTCGTCTCGCGTCGCCCGCTCGACGCGGCGGGCGCTGGCCT
GCTGGCCGAACTCAACGACCTCGGCGCGGAAGCGGTGCATGTGCGCGCCGACGTGTCGGTCGCCGCCGACGCCGAGCGCGTCGCCGCGATCGCCCACGCC
ACATTCGGGCCGGTCGACGTGCTCTACCACGGCGCGGGACAGCTGCGCGACGGTCTGGTGTCCGGCAGCGGCGGCGACGAAGCGATTTCGGTTCTGGCGC
CGAAGGTGCACGGCGTGCGGCACCTGACCCGCGCGCTGCCGGAAGCCCGGCTGACGGTGTTGTTCTCCTCGGTCTCCGCGGTGCTCGGCAATCCGGGGCA
GGCGGACTACGCGGCGGCCAACCGCTACCTCGACGTCTTCGCCGAACAGCGTGACCAGGCCGGAACCAGGACGGTCAGCGTCAACTGGCCGCTGTGGGCC
GAGGGCGGCATGCATCCACCCGCCGAGCAGATCGCCGCGCTGCGGCGCGCCGGGATCGGACTGCTGCCCACCGCGACCGGACTCGACCTGCTGCTGTCGT
GCGCCGCGCTGGACCGCGCCCAACTCGCCGCGGTCTGGGGCGACCCCGAAGTCGTGCGAGCCCGGCTGCGTCCGGCCGCCTCGCAGGCACGTGCGGCCGT
CGAGTCGGTGACCGCCGACGAGCATCCTGCCCCGGCGAGACCGGTCGGCGTCGAGGAGCCGATCGCGATCGTCGGCGTCAGCGGACGCTACCCGGACGCC
GACGACCTCGACGAGTTCTGGCGCAATCTGCGCGACGGCCACGACGCGGTCGGCCCGGTCCCCGCGGAACGCTGGTGGCTCACCGCCGCCCGAGACACCC
CCGGCGCACCGCCGATGACCGGCGGCTTCCTGCGTGACGTAGGCGGTTTCGACCCCCAGTTCTTCCGCATTCCGCCCGTGCGGGCGCCACTGCTGGATCC
GCAGGAGCGGCTGTTCCTGCAACAGGCGTATGCCGCACTGGAACACGCGGGCTACCACCACACCGACCCGAGTGTGCGGGATCGGCGCGTCGGTGTATTC
GTCGGCGCCACCTGGAGCGACTACCGGCTGCTGGGCGTGGAGGCCAGCCGCGACGGCGATCCGGTCGCCGTGGCGTCGATTCCGTCGTCCATCGCCAACC
GCACCTCCTACACCCTCGGCCTGCGCGGACCGAGTCTGACCGTGGACACCGCCTGCTCGGCATCGCTGACCGCTCTGCACCTGGCGCGGCGCGCCATGCT
GGATGGCGAATGCGACGCCGCGATCGTCGGCGGCGTGAATCTGCTGCTGCACGAGGACAAGTTCCTGCTGCTGAGCAGCCTGGGCATGATGTCGGGCAGC
GGCCGGTGTCGGCCGTTCGCCGCGAGCGCCGACGGGTACGTCCCGGGCGAAGGCGTCGGCGCGCTGTATCTGAAACCGCTGTCCGCCGCCCGCGCCGACG
GCGACACCATCCACGGACTGATCCTGGGCAGCGCCGTCAATCACGGCGGCCAGACCTCCGGATACAGCGTGCCGCACCCCGGCGCGCAGGCCGATGTGAT
CACCGAGGCGCTGCGTGCCGCGGGGGTCGACCCGGCGGCGATCGACTACCTGGAGGCGCACGGCACCGGAACGCCGCTCGGCGATCCGATCGAAATCGCC
GGTCTCGCCGCGGCATTCGGCCGATCCGACGGAACCGGCCGCTCCCGCGCGCTCGGGTCGACCAAATCGAACATCGGCCACCTGGAAGCGGCCGCGGGCG
TCGCGGGGCTGACCCGCGTGCTCTTGCAGATGCGGCACGGCCGGATCGCGCCGACGCTGCACGCGGCACAGGTCAATCCGGCGATCGCGTTCGACTCGGT
GCCGTTCCATCTGCCGCGCGAGCTGGAGGAATGGCCGCGACGCACGGTCGACGGCGTCACGGTGCCGCGGTATGCCGGTGTCAGCGCGTTCGGTGCGGGC
GGAGCCAACGCCCACGTGGTGCTCGCCGAATTCGTCGACGACCGAACTCGCTCCCGGATGCCGGTTGCGCCGGAGATCTTCGTGCTCTCGGCACGCACGG
CGGCCCAATTGCGCCGCTACGCCGAGATCATGGCGCGGCACTTCCGCACGCTCAACCCGGCCCATTTCACCGACTCGGTGCGCACTTTGCAGGTGGGCAG
GCCCGCGCTCGCACACCGGATGGCGGTGGTCGCGTCATCACCGGTCGCGGTGGCCGACGCGCTCGACCAGTTCCTCACCGGCCAGCCGGGTCCGGTGCTG
ACCGGCACAGCGGACGCGCAGACCCGTCCACAGTTCGGCCCACCGGCGAGCGATCAGGACCTGGCCCGCCATTGGGTCGCGGGCGCCGACATCGATTGGC
GTGACCGCGGCGGCCCCGAACGACGAGTTGCATTGCCCGGCTATCCCTTCGATCGCACCGACTACTGGATCCTCACGCCGCCGCAGTCCACGTTCGACGC
CGCGGTGGCGCAATTCGAGGCCGCGACCCGGTCCGCGGGCGTCGTCGAGCCCGGCAAGCCCGAGCTCGGCCGGTACGCGGCGATGCTCCTGTTCGCCACG
CTGGCCGGGATGGGTCTCACGCCAGGGGAGTGGACCGTCGAGTCGCTGGACGCGGCGCTGGGCGTGCGGCCGGAATTCGCGCGGTTGCGCACCAGCTGTC
TGGACATTCTGCTGCGGCACGGGCTCCTCTGCTCGCAGCAGGACACCATCGTCGTGCCCGGCCCGCCTCCCGGCACCAGCGGTATGCGCGCCGACCTGCT
CGCGCGCTTCCCGGAGACCAAGGCGTATGTGACGCTGCTCGACGCCTGCCTGGCCGCGTACCCTCGTGTGCTGCGCGGGGAGCTGTCGGCCACGGAGGTG
CTGTTCCCCGGCGGCGACCTCGAGCTGGTCGGCGCGATCTATCGCGGCAACGCCGCCTACGATTTCACCAACGGCCTGCTCGCCGAATTGGTGTCGGCTC
TCGCGCGGCAGCGCGGCACCGCGCGCCCCCTGCGGATTCTCGAGATCGGCGCGGGCACCGGCGGTTCCACGCACGCGGTGCTCGCAGCACTCACCGAACA
TGGGACGGCGTACGAGTACCACTACACCGACGTCTCGCACAGTTTCGTCGCGCACGGACGGCGCACCTTCGGCACAAACCCGAACCTGCGTTTCACCGTG
CTGGACATCGAGACCGATCCGGTCGCGCAAGGGTTCGCCGCGGGCGACGCCGACCTGGTGGTGTGCGCCAACGTCCTGCACGCCACGCGGTCGATCGACT
CGACACTGCGCAACGCGCGACGGCTGCTGGCACCGGGCGGCGTGCTGGCGTTCACCGAGGCGACCACCGATCTGGAGGTGCTCGCACTGACCTTCGGGCT
GCTGGAGGGCTGGCACGCCTACCGCGATCCCGCGGCGCGAATCCCGCACTCGCCGTTGCTCTCCGAGCAACAGTGGCGCGACGCCTTGAGCGCGGCGGGA
TTCTCCGGAATCCGGGCGTGGGGTCCGGGCCTGTCCGAGGACCCGGAACCGAGCTTCCGGCTGCTGGCCGCCGTCAGCGCCGCCGCGCCCGCCGATAGCC
GGTCGGAACCCGCTGTCTTCCCGGAACCGGCGCGGGCCGACGCGGTCGGCGCATCCGCGCAGTTCGCCGACAATCCGGCGGGAAGTGTGGGACGCCTCGA
CTCGGAACCCGCGCGGACAGGAGCGGCGTCCGCACCCGCATCGTCTGCTGCTGCCTCGGCGCTGGAAGCCGAGCTCATCGGCCTCGTCGCCGACGGACTC
GGCGTCGCGCACTCCGAAGTGCGCGCCGAGTTGCCGCTGTCGGAGTACGGCGTCGATTCGATCCTAGCGGTGAAGATCATCGACCGCGTCAACGCCCGCT
ACGGCTTGGCGCTGCGGCCGACAGTGGTGTTCGACCATCCGTCCGTGCGACTGCTCAGCAGGCGTGCCGAAGACGGCGGCACCGAGGTCCGGGGGGAGGC
GCCGTCGGCCCTCGCCGGGCTGGACGCGCTGGAAGCGGCCCTGCCCGAGGTGCCCGCAACCGAGCGGGAAGAGCTGGTACAGCGCTTGGAACGGATGCTC
GCCGCGCTACGCCCGGTCGCCCAGGCCGCCGACGCCTCCGGGACCGGCGCCAACCCGTCCGGCGACGACCTGGGCGAGGCGGGCGTGGACGAACTGCTCG
AAGCACTCGGCCGGGAGCTCGACGGCGATAAACTTGGATCGTGGAGCCACCCGCAGTTCGAAAAATAACTAGGCTGCTGCCACCGCTGAGCAATAACTAG
CATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAACCTCAGGCATTTGAGAAGCACACGGTCACACTGCTTCCGGTAGTCAATAA
ACCGGTAAACCAGCAATAGACATAAGCGGCTATTTAACGACCCTGCCCTGAACCGACGACCGGGTCATCGTGGCCGGATCTTGCGGCCCCTCGGCTTGAA
CGAATTGTTAGACATTATTTGCCGACTACCTTGGTGATCTCGCCTTTCACGTAGTGGACAAATTCTTCCAACTGATCTGCGCGCGAGGCCAAGCGATCTT
CTTCTTGTCCAAGATAAGCCTGTCTAGCTTCAAGTATGACGGGCTGATACTGGGCCGGCAGGCGCTCCATTGCCCAGTCGGCAGCGACATCCTTCGGCGC
GATTTTGCCGGTTACTGCGCTGTACCAAATGCGGGACAACGTAAGCACTACATTTCGCTCATCGCCAGCCCAGTCGGGCGGCGAGTTCCATAGCGTTAAG
GTTTCATTTAGCGCCTCAAATAGATCCTGTTCAGGAACCGGATCAAAGAGTTCCTCCGCCGCTGGACCTACCAAGGCAACGCTATGTTCTCTTGCTTTTG
TCAGCAAGATAGCCAGATCAATGTCGATCGTGGCTGGCTCGAAGATACCTGCAAGAATGTCATTGCGCTGCCATTCTCCAAATTGCAGTTCGCGCTTAGC
TGGATAACGCCACGGAATGATGTCGTCGTGCACAACAATGGTGACTTCTACAGCGCGGAGAATCTCGCTCTCTCCAGGGGAAGCCGAAGTTTCCAAAAGG
TCGTTGATCAAAGCTCGCCGCGTTGTTTCATCAAGCCTTACGGTCACCGTAACCAGCAAATCAATATCACTGTGTGGCTTCAGGCCGCCATCCACTGCGG
AGCCGTACAAATGTACGGCCAGCAACGTCGGTTCGAGATGGCGCTCGATGACGCCAACTACCTCTGATAGTTGAGTCGATACTTCGGCGATCACCGCTTC
CCTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATA
GCTAGCTCACTCGGTCGCTACGCTCCGGGCGTGAGACTGCGGCGGGCGCTGCGGACACATACAAAGTTACCCACAGATTCCGTGGATAAGCAGGGGACTA
ACATGTGAGGCAAAACAGCAGGGCCGCGCCGGTGGCGTTTTTCCATAGGCTCCGCCCTCCTGCCAGAGTTCACATAAACAGACGCTTTTCCGGTGCATCT
GTGGGAGCCGTGAGGCTCAACCATGAATCTGACAGTACGGGCGAAACCCGACAGGACTTAAAGATCCCCACCGTTTCCGGCGGGTCGCTCCCTCTTGCGC
TCTCCTGTTCCGACCCTGCCGTTTACCGGATACCTGTTCCGCCTTTCTCCCTTACGGGAAGTGTGGCGCTTTCTCATAGCTCACACACTGGTATCTCGGC
TCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTAAGCAAGAACTCCCCGTTCAGCCCGACTGCTGCGCCTTATCCGGTAACTGTTCACTTGAGTCCAACC
CGGAAAAGCACGGTAAAACGCCACTGGCAGCAGCCATTGGTAACTGGGAGTTCGCAGAGGATTTGTTTAGCTAAACACGCGGTTGCTCTTGAAGTGTGCG
CCAAAGTCCGGCTACACTGGAAGGACAGATTTGGTTGCTGTGCTCTGCGAAAGCCAGTTACCACGGTTAAGCAGTTCCCCAACTGACTTAACCTTCGATC
AAACCACCTCCCCAGGTGGTTTTTTCGTTTACAGGGCAAAAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACTGAACCGC
TCTAGATTTCAGTGCAATTTATCTCTTCAAATGTAGCACCTGAAGTCAGCCCCATACGATATAAGTTGTAATTCTCATGTTAGTCATGCCCCGCGCCCAC
CGGAAGGAGCTGACTGGGTTGAAGGCTCTCAAGGGCATCGGTCGAGATCCCGGTGCCTAATGAGTGAGCTAACTTACATTAATTGCGTTGCGCTCACTGC
CCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTC
TTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAA
ATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATGAGCTGTCTTCGGTATCGTCGTATCCCACTACCGAGATGTCCGCACCAACGCGCAGCCCGGAC
TCGGTAATGGCGCGCATTGCGCCCAGCGCCATCTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCCCTCATTCAGCATTTGCATGGTTTGTTGAA
AACCGGACATGGCACTCCAGTCGCCTTCCCGTTCCGCTATCGGCTGAATTTGATTGCGAGTGAGATATTTATGCCAGCCAGCCAGACGCAGACGCGCCGA
GACAGAACTTAATGGGCCCGCTAACAGCGCGATTTGCTGGTGACCCAATGCGACCAGATGCTCCACGCCCAGTCGCGTACCGTCTTCATGGGAGAAAATA
ATACTGTTGATGGGTGTCTGGTCAGAGACATCAAGAAATAACGCCGGAACATTAGTGCAGGCAGCTTCCACAGCAATGGCATCCTGGTCATCCAGCGGAT
AGTTAATGATCAGCCCACTGACGCGTTGCGCGAGAAGATTGTGCACCGCCGCTTTACAGGCTTCGACGCCGCTTCGTTCTACCATCGACACCACCACGCT
GGCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGACTGGAGGTGGCAACGCCAATCAGCAACGACTGT
TTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCT
GGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTC
TTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCCGGGATCTCGACGCTCTCCCTTATGCGACTCCTGCATTAGGAAAT;

https://benchling.com/s/seq-eaRmq6aHEQcqsjsegdT5