Kevin T.L. Vogelsang
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| Plate | Expected | Observed |
|---|---|---|
| 1) (-) No DNA | none | none |
| 2) (+) untreated plasmid | lots | 624 |
| 3) backbone + kilcut (- ligase) | none | none |
| 4) backbone + kilcut (+ ligase) | none | none |
| 5) backbone +kilcut +ligase + insert | lots | 7 |
| Transformation Efficiency: (1.25 e5) colonies per microgram |
Ubp8
Forward Primer: uppercase: Ubp8 tail italicized lowercase: landing region
TACTTGAAACCCTGCTTTTTTTATTTGTTATTAATAATTatgtcgaaagctacatataa
Top strand of full ‘reverse’ primer: capital: tail italicized lower case: landing sequence 5’ TTCAGCAAATAGCATTCAACAATAATAATAAAACAAAAAgatgcggccagcaaaactaa3’
