KAD072406-Construction of pSB1A2-J01129-J01007

From OpenWetWare
Jump to navigationJump to search

[pK2(presumably on pSB1A2)]+[key3]
Digest pSB1A2-J01007 [AlwnI/SpeI, Neb2, Large-1593]
Paste into pSB1A2-J01129 [AlwnI/XbaI, Neb2, Small-710bp]
Product is pSB1A2-J23024
this construction has been abandoned because it turns out that we dont have the basic part for key3. the construction file here describes the construction of [pK2][Ptet][key3] which is stupid.