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Tahoura Samad 13:22, 2 July 2010 (EDT)
- most of my transformations with the new competent cells were successful.
|Competent cell||Number||PDD||Growth in initial competent cells||Growth in Small Scale Competent Cells||Result of Assay with Jin||Conclusion|
|pBca156-Bjh2343||1||2331||0||tons of colonies||NOT DONE||transform new, assay|
|pBca156-Bjh2343||1||2333||0||tons of colonies||NOT DONE||transform new, assay|
|pBca156-Bjh2343||1||2348||1 colony (twice)||tons of colonies||NOT DONE||transform new, assay|
|pBca156-Bjh2343||1||2349||0||50-100 colonies||NOT DONE||transform new, assay|
|pBca156-Bjh2343||1||2291||0||100-200 colonies||NOT DONE||transform new, assay|
|pBca156-Bjh2343 with BAC||7||2331||0||15 colonies||NOT DONE||Today|
|pBca156-Bjh2343 with BAC||7||2333||0||2 colonies||NOT DONE||Today|
|pBca156-Bjh2343 with BAC||7||2348||0||4 colonies||NOT DONE||Today|
|pBca156-Bjh2343 with BAC||7||2349||0||0||DEAD||\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\|
|pBca156-Bjh2343 with BAC||7||2291||0||0||DEAD||\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\|
|pBca156-Bjh2344||2||2331||0||tons of colonies||NOT DONE||make new, transform, assay|
|pBca156-Bjh2344||2||2333||0||tons of colonies||NOT DONE||make new, transform, assay|
|pBca156-Bjh2344||2||2348||12 colonies||TO DO||V (UNSTABLE)||make new, transform new and old, assay|
|pBca156-Bjh2344||2||2349||6 colonies||TO DO||V V||make new, transform new and old, assay|
|pBca156-Bjh2344||2||2291||7 colonies||TO DO||V V||make new, transform new and old, assay|
|pBca156-Bjh2344 with BAC||6||2331||tons of colonies||X||X||\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\|
|pBca156-Bjh2344 with BAC||6||2333||tons of colonies||X||X||\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\|
|pBca156-Bjh2344 with BAC||6||2348||1 colony (twice)||100-200 colonies||NOT DONE||Today|
|pBca156-Bjh2344 with BAC||6||2349||1 colony(twice)||35-50 colonies||X||\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\|
|pBca156-Bjh2344 with BAC||6||2291||35-50 colonies||100-200 colonies||V V V||transform new, assay|
|pBca156-Bjh1934||3||2348||tons of colonies||X||V V||transform old, assay|
|pBca156-Bjh1934||3||2349||tons of colonies||X||V V||transform old, assay|
|pBca156-Bjh1934||3||2291||tons of colonies||X||V||transform old,assay|
|pBca156-Bjh1934 with BAC||5||2331||tons of colonies||X||X||\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\|
|pBca156-Bjh1934 with BAC||5||2333||tons of colonies||X||X||\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\|
|pBca156-Bjh1934 with BAC||5||2348||tons of colones||X||X||\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\|
|pBca156-Bjh1934 with BAC||5||2349||1o colonies||X||X||\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\|
|pBca156-Bjh1934 with BAC||5||2291||tons of colonies||X||V V V||transform old, assay|
- competent cell checks for new 1 and new 6 looked good.
- miniprepped IBB as CA lefty, CK lefty and CK righty, and sent out for sequencing with ca998.
Tahoura Samad 13:38, 1 July 2010 (EDT)
- controls for JTK030 looked good with no growth on Kan, Amp, Cam, good growth on LB, and a little growth on Spec, which is the normal result because of mutations in the bacteria. Those are labeled with double black stripes, in a box in the back of the -80 in the 2nd row from the top.
- picked and grew up the results of PCA for the new nuclear localization tag. They had plenty of colonies, with only one background green colony. Will miniprep them tomorrow.
- did small scale competent cell prep of 1 and 6.
- did the following transformations (1old:2348), (5 old:2291) (6 old:2291) (6 new:2291),(3 old :2348) (3 old: 2349) (3 old:2291) (2 old:2348) (2 old: 2349)(2 old:2291) (2 new:2348) (2 new:2349) (2 new: 2291), (1 new:2331) (1 new:2333) (1 new:2348) (1 new:2349) (1 new: 2291) (6 new 2348) (6 new:2349), (IBB into lefty).
- redid assembly of stage 0 of life act but with 2.4 μL of each DNA and 1.2 μL of 5x NEB2.