July

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Tahoura Samad 13:22, 2 July 2010 (EDT)

  • most of my transformations with the new competent cells were successful.
Competent cell Number PDD Growth in initial competent cells Growth in Small Scale Competent Cells Result of Assay with Jin Conclusion
pBca156-Bjh2343 1 2331 0 tons of colonies NOT DONE transform new, assay
pBca156-Bjh2343 1 2333 0 tons of colonies NOT DONE transform new, assay
pBca156-Bjh2343 1 2348 1 colony (twice) tons of colonies NOT DONE transform new, assay
pBca156-Bjh2343 1 2349 0 50-100 colonies NOT DONE transform new, assay
pBca156-Bjh2343 1 2291 0 100-200 colonies NOT DONE transform new, assay
pBca156-Bjh2343 with BAC 7 2331 0 15 colonies NOT DONE Today
pBca156-Bjh2343 with BAC 7 2333 0 2 colonies NOT DONE Today
pBca156-Bjh2343 with BAC 7 2348 0 4 colonies NOT DONE Today
pBca156-Bjh2343 with BAC 7 2349 0 0 DEAD \\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\
pBca156-Bjh2343 with BAC 7 2291 0 0 DEAD \\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\
pBca156-Bjh2344 2 2331 0 tons of colonies NOT DONE make new, transform, assay
pBca156-Bjh2344 2 2333 0 tons of colonies NOT DONE make new, transform, assay
pBca156-Bjh2344 2 2348 12 colonies TO DO V (UNSTABLE) make new, transform new and old, assay
pBca156-Bjh2344 2 2349 6 colonies TO DO V V make new, transform new and old, assay
pBca156-Bjh2344 2 2291 7 colonies TO DO V V make new, transform new and old, assay
pBca156-Bjh2344 with BAC 6 2331 tons of colonies X X \\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\
pBca156-Bjh2344 with BAC 6 2333 tons of colonies X X \\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\
pBca156-Bjh2344 with BAC 6 2348 1 colony (twice) 100-200 colonies NOT DONE Today
pBca156-Bjh2344 with BAC 6 2349 1 colony(twice) 35-50 colonies X \\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\
pBca156-Bjh2344 with BAC 6 2291 35-50 colonies 100-200 colonies V V V transform new, assay
pBca156-Bjh1934 3 2331 35-50 colonies X X \\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\
pBca156-Bjh1934 3 2333 <100 colonies X X \\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\
pBca156-Bjh1934 3 2348 tons of colonies X V V transform old, assay
pBca156-Bjh1934 3 2349 tons of colonies X V V transform old, assay
pBca156-Bjh1934 3 2291 tons of colonies X V transform old,assay
pBca156-Bjh1934 with BAC 5 2331 tons of colonies X X \\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\
pBca156-Bjh1934 with BAC 5 2333 tons of colonies X X \\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\
pBca156-Bjh1934 with BAC 5 2348 tons of colones X X \\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\
pBca156-Bjh1934 with BAC 5 2349 1o colonies X X \\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\
pBca156-Bjh1934 with BAC 5 2291 tons of colonies X V V V transform old, assay
  • competent cell checks for new 1 and new 6 looked good.
  • miniprepped IBB as CA lefty, CK lefty and CK righty, and sent out for sequencing with ca998.

Tahoura Samad 13:38, 1 July 2010 (EDT)

  • controls for JTK030 looked good with no growth on Kan, Amp, Cam, good growth on LB, and a little growth on Spec, which is the normal result because of mutations in the bacteria. Those are labeled with double black stripes, in a box in the back of the -80 in the 2nd row from the top.

IMG 5128.JPG Kan
IMG 5129.JPG LB
IMG 5130.JPG Cam
IMG 5131.JPG Amp
IMG 5132.JPG Spec

  • picked and grew up the results of PCA for the new nuclear localization tag. They had plenty of colonies, with only one background green colony. Will miniprep them tomorrow.
  • did small scale competent cell prep of 1 and 6.
  • did the following transformations (1old:2348), (5 old:2291) (6 old:2291) (6 new:2291),(3 old :2348) (3 old: 2349) (3 old:2291) (2 old:2348) (2 old: 2349)(2 old:2291) (2 new:2348) (2 new:2349) (2 new: 2291), (1 new:2331) (1 new:2333) (1 new:2348) (1 new:2349) (1 new: 2291) (6 new 2348) (6 new:2349), (IBB into lefty).
  • redid assembly of stage 0 of life act but with 2.4 μL of each DNA and 1.2 μL of 5x NEB2.