Jonesma Lab:Protocols:ggmod

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Golden Gate Ligations

Follow Nicola Patron's startup guide. If there are internal BsaI or BpiI sites add these additional steps to the end:

1) Add 1.5 μL T4 ligase buffer and 0.5 μL T4 Ligase

2) Incubate at 16°C for 1 hour

3) Heat to 65°C for 10 min

4) Transform into Top10 cells