Jonesma Lab:Protocols:gelelectrophoresis

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  1. Make a 1% agarose gel (0.8g agarose in 80mL dH2O
  2. Microwave to melt agarose (Careful! 1min is usually sufficient)
  3. Wait for agarose to cool (~10min)
  4. Add 2.4μL SafeView dye (to stain DNA)
  5. Pour gel
  6. Add 4μL of loading dye (pink) to 20μL of DNA sample
  7. Load gel
  8. Run at 100V for 30min
  9. Image gel- remember to save for your report!