Jessica Karen Wong/Notebook/2007-8-5

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  • Overnight plates looked good
  • Set up colony pcr's of F2620 and E0240
    • 4 of each of the 3 F2620-3K3 plates
    • 4 of each E0240-M1-ES, E0240-M2-ES, E0240-E2-ES, E0240-E2-EX, E0240-M2-EX, E0240-EX-E1
    • 2 of each E0240-ES-E1, E0240-EX-M1
  • Minipreped B0034
  • Digested B0034 with each E/S, X/S
  • Ran gels of the overnight colony pcr
Large Gel
  • Large gel:
    • Row 1 - I2055-SNX (8-1) [chose 3], ladder, I2055-ENX-(8-1) [chose 3],

I2057-EX-J23100 (4)

    • Row 2 - I2057-EX-J23100 (3-1) [chose 2], I2057-EX-J23116 (1-4), ladder

[chose 1], I2057-EX-R0040 (4-1) [chose 1], I2057-ES-R0040 (1-4) [chose 3], I2055-SX-B0031 (1,2) [chose 1]

Small Gel
  • Small gel:
    • row 1 - I2055-SX-B0031 (3,4), I2055-SX-B0032 (1-4) [chose 2], ladder,

I2055-EX-B0032 (1,2)

    • row 2 - I2055-EX-B0032 (3,4) [chose 3], I2055-SX-B0030 (1-4) [chose 2]