Jessica Karen Wong/Notebook/2007-8-5
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- Overnight plates looked good
- Set up colony pcr's of F2620 and E0240
- 4 of each of the 3 F2620-3K3 plates
- 4 of each E0240-M1-ES, E0240-M2-ES, E0240-E2-ES, E0240-E2-EX, E0240-M2-EX, E0240-EX-E1
- 2 of each E0240-ES-E1, E0240-EX-M1
- Minipreped B0034
- Digested B0034 with each E/S, X/S
- Ran gels of the overnight colony pcr
- Large gel:
- Row 1 - I2055-SNX (8-1) [chose 3], ladder, I2055-ENX-(8-1) [chose 3],
I2057-EX-J23100 (4)
- Row 2 - I2057-EX-J23100 (3-1) [chose 2], I2057-EX-J23116 (1-4), ladder
[chose 1], I2057-EX-R0040 (4-1) [chose 1], I2057-ES-R0040 (1-4) [chose 3], I2055-SX-B0031 (1,2) [chose 1]
- Small gel:
- row 1 - I2055-SX-B0031 (3,4), I2055-SX-B0032 (1-4) [chose 2], ladder,
I2055-EX-B0032 (1,2)
- row 2 - I2055-EX-B0032 (3,4) [chose 3], I2055-SX-B0030 (1-4) [chose 2]