Jeff Tabor/MEGAWHOP
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Reaction setup
- 120ng template plasmid
- 500ng Megaprimer
- Pfu Ultra II
Cycling conditions
95C, 2'
95C, 20"
55C, 20"
68C, 6-12'
25x
Transformation
- DpnI, 1-2h
- Qiaclean the reaction
- Transform 0.5-2uL clean DNA into 50uL chemically competent E.coli
- Plate all
