Jasieniuk:Notebook/Apomixis in hybrid Rubus/2010/09/13
Apomixis in hybrid Rubus | Main project page Previous entry Next entry |
GeneMapper allele calling
In this file, do-overs have been consolidated with the original runs. All of the seed samples from April-May are in this file because I haven't had the time yet to go through them and figure out which are the failed samples.
- 1: Seed well plate file also corresponds to those plates. A means plate 1, B means plate 2. Samples labeled "L" are the most recent leave samples, and if they are labeled "S" then those are the old seed runs, which are different from the current. In the current run, I did not do sample set name "BWP" because they were 80+% successful on the old seed run, and I still keep the old BWP dilution plate in the freezer. - 2: In Genemapper, I admit I also saw allele in the H, and those are indeed blanks (probably spilled), and they are not parents. - 3: The parent individuals are in the older leaf dilution plates, but not in the seed plates.
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