JCA062306-Construction of pJ23004
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PCR ca1002F/R on pSB1A3-I13521 (987 bp, EcoRI/PstI, Neb2)
Sub into pSB1A3-I13521 (EcoRI/PstI, Neb2)
Product is pJ23004
ca1002F Forward EcoRI oligo to make XbaI blunter cassette
cttctggaattcgcggccgcaactagtgtccctatcag
ca1002R Reverse PstI oligo to make SpeI blunter cassette
gaagcctgcagcggccgctTctagAatataaacgcag
