JCA061306-Construction of pSB1A2-J23000
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[pMB1][AmpR][StrRBS][TraJF]+[dblTerm]
digest pSB1A2-J01052 (SpeI/AlwNI, Neb2, Large-2241)
digest pSB1A2-B0015 (XbaI/AlwNI, Neb2, Small-546)
Ligate, Product pSB1A2-J23000
