Initial Testing for DNA constructs for CELL BY DATE In Vitro

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Aims:

  • To determine which constructs for cell by date expresses in vitro
  • To test the pTet and pcI constructs in commercial S30 E.coli cell extract and home made S30 extract
  • To test the pT7 construct in commercial S30 T7 cell extract and home made S30 extract.
  • To investigate optimum volume of home made S30 extract
  • To investigate the differences between home made and commercially bought S30 extract. This is in terms of rates of expression, length of expression and total output.