Infinite monkey group meeting 31 Oct 2008
Ton: Tutorial on DNA editing enzymes. Presentation: writing to DNA
Carolina: Application of memory devices to Aging and developmental biology.
(Please feel free to edit if you have answers...)
1. How is the E.coli DNA polymerase I targeted to specific origins?
- Could imagine targeting and error-prone polymerase to a repressor (or other inhibiting gene), and have its mutation then lead to expression of the next polymerase. Would this be a primitive write-once daisy chain memory?
2. Note that Sangamo Inc has some zinc finger targeted nuclease papers in the literature too.
- PMID 15806097, Highly efficient endogenous human gene correction using designed zinc-finger nucleases.Nature. 2005 Jun 2;435(7042):646-51. Epub 2005 Apr 3.
- Note, could again imagine daisy-chaining trans-correcting zinc finger nucleases
3. Naive background question... are there subclasses of recombinases, one of which are destructive of targeting sites while the other is non-destructive?
4. What is a realistic estimate for number of Tn3-like serine recombinases? Same deal for the phage integrases? I.e., how many independent/orthogonal enzymes might be found in nature?
5. Note the DSCAM protein from fly brain wiring. Single protein that can exist in over 2^15 forms.
- See this URL for info (but also look
for a recent review): http://www.hhmi.org/news/zipursky.html
- original article: link
- a review on Dscam: link
6. Note #5 might lead us to consider RNA or proteins for storage
7. Are there telomere-related aging issues in yeast? Or, issues of chromosomal stability?
8. Is there a regulated transition from asymmetric to symmetric to asymmetric division, in yeast?
9. Has anybody tried to express (in yeast) a daughter specific lethal gene (e.g., cbk1)?
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