Imported:YPM/Ptc1

• Ptc1 phosphatase is recruited to Ste5 upon pheromone stimulation and dephosphorylates Ste5 T287. This dephosphorylation competes with Fus3 phosphorylation of the same site. Dephosphorylation of Ste5 is required for Fus3 to be activated by Ste7 and dissociate from Ste5. Malleshaiah et al 2010 doi:10.1038/nature08946

• 2-fold overexpression of Ptc1 increases Fus3 biphosphorylation inducibly without significant effect on basal induction. Malleshaiah et al 2010 doi:10.1038/nature08946