ISISBio:Internal/Lab Supplies/old

Tues 26th August

• ELGA UHQ5 RO cartridge, Cooling Fan, 1/2 day labour Quote WQ005499

Thurs 21st Aug

• New England Biolabs BlpI R0585S 500 units 10,000 units/ml
• Agilent - XL1 Blue Competent Cells 5 x 0.2ml ordered 2 packs
• Promega BamH1 restriction enzyme
• Fisher 5ml pipette tips
• Jencons Jenway pH Meter expected end of August
• 100pk 0.22um 25mm filter membranes

Weds 20th Aug

• BioRad Precision Plus Protein Kaleidoscope standards x2

Date unknown

• Bugbuster - ===arrived?===
• Benzonase (from Novagen as well as bugbuster) ===arrived?===
• Glucose (if we don't have some but I don't think we do) ===arrived?===

• Fisher kilobase marker x2 one in stock one to follow
• Fisher 25-500bp marker x2
• Fisher Invitrogen Kanamycin sulphate 25g
• NdeI and NaeI (Promega R7131 and R6801)
• Merck dodecyl maltoside 25g

Mon 11th August 2008

• Lyreco 10x Arch files for Louise's office R78
• Whiteboard markers 1 x 4pk
• Whiteboard cleaning and accessory kit
• A4 ruled manuscript books x5

Fri 8th August 2008

• Fisher by requisition fume hood and cabinet

• items ordered Weds 6th August
• Fisher Sodium Acetate 500g
• 10ml syringes
• 30ml Syringes
• Paper rolls for lab
• Fisher 25g Arabinose
• 10pk 500ml Durans
• 10pk 1L Durans
• 10pk 250ml Durans
• 5x 1ml Histrap Ni columns
• ordered weds 30th July 2008 25g dodecyl maltoside (DM)
• Weds 9th July 2008 arrived Heraeus Megafuge 11R, 230V Ex-Demo unit s/n 306090617 Delivery expected 11/08/08
• T41 Swing out rotor
• Set of 4 buckets for T41 rotor
• Sealing lids for T40 buckets
• Insert, 1 x 280ml (64 x 113mm)
• Insert, 2 x 50ml (29 x 114mm)
• Delivery BM sited
• NH1/070 Custom underneath trolley delivery expected 31/08/08

Fri 1st August

• GE Healthcare XK16/20 column x1
• GE Healthcare 16/20 tube x1
• NEB T4 DNA ligase x1
• NEB Vent Polymerase x1

Weds 30th July

• Fisher DTT x2
• Fisher Cuvette Uvette 80 disposable single sealed x1 pack

Mon 28th July

• ELGA service contracts and 6 month supplies
• BioRad Short Plates, 5 2packs
• BioRad Spacer Plates with 0.1mm integrated spacers 1pack

Tues 16th July 2008

• Fisher NaOH 1M
• Epp tips 2ul-200ul
• Epp tips 0.1ul-20ul
• Epp tips 100ul-1000ul

Mon 14th July 2008

• HP Printer/copier/scanner and two colour cartridges -

Mon 7th July

• Fisher LAB-850-112N Biohazard sign x4
• Fisher LAB-850-113L Biohazard rigid sign x1
• Fisher LAB-830-176B Now Wash hands sign x2
• Fisher LAB-835-034X Hazardous Area sign x2
• Fisher LAB-850-086L Flammable gas sign x1
• ARCO Ergoclean Mop box with lid

Thurs 3rd July

• NEB T4 DNA Ligase x2
• NEB Vent DNA Polymerase

Tues 1st July

• Fisher AKL-300-030M Foil 450mm x 75M x2
• Fisher AUY-200-030K 9.5L sterilising pan x1
• Fisher AUY-220-070H 3.1L sterilising pan x1
• Fisher BPE-1760-25 Ampicillin sodium salt 25g x2
• Fisher CRY-580-010X Cooler labtop for 12 tubes x1
• Fisher FB55924 Cuvette UV single use 1.6ml 1 box
• Fisher FB56209 Gel loading tips 1-200ul 1 box
• Fisher FB71251 Cryobox with cardboard lid white 32mmx32mm 2packs
• Fisher PDT-100-010M Incubation tray for petri dish
• Fisher PMP-890-511T 100ul-1000ul pipettor tips eppendorf 5 boxes
• ARCO Fliptop bin blue 54L x1
• ARCO Fliptop bin red 54L x1
• Ergoclean mop handle 2.5M long x1
• Ergoclean Wet multifibre mix head x1

26th June

• Amazon HP Ink Cartridge Tricolour x2
• Amazon Photosmart C3180 All-in-one Printer

24th June

• Fisher SAT-670-010M Spillage clean up 3M chemical sorbent sheets 4 boxes
• Perbio from Thermofisher 23225 Bradford Assay Kit 500rx x1

23rd June

• Merck 324355 DM 25g
• Promega R6801 Nde1
• Promega R7131 Nae1
• Fisher BPE-2553-100 Kilobase Marker
• Fisher BPE-LAD4-200 DNA MW Markers 25-500bp
• Fisher Invitrogen VX11815032 Kanamycin Sulphate
• Bradford reagent or stuff to make it - have made enquiries. Should be ordered Fri 13th.
• Welded 2 tier trolley
• Sterile pipettes 5ml
• Sterile pipettes 10ml
• Sterile pipettes 25ml
• Sterile pipettes 50ml
• Rhamnose (sugar - Fisher 17408-0250 for 25 g)
• BioRad- Bio-Safe Coomassie Stain, 5L x1 A box with a tap on for easy dispensing

• BioRad- Precision Plus Kaleidoscope Standards 500ul (50 applications at 10ul per well) x2 This is a rainbow marker which I used at Reading Uni and was brilliant because there is never a doubt about the size of each marker band. I'v ordered 2 so we can use one and when that is used up we re-order so we never run out. To be stored at -20 when not in use. Store at room temp or in cold room when in use.

• Syringe filter sterile 0.2um 25mm
• Syringe filter sterile 0.2um, 4mm
• Vivaspin protien concentrators 0.1-0.5ml
• Merck Dodecyl Maltoside 5g, overnight shipment requested
• -80 freezer storage-fisher

Weds 4th June

• Ep tips box 0.1-20ul

===MAY

Thurs 29th May

• Fisher Revco racking x3

Fri 23rd May

BioRad Biosafe Coomassie Stain 5L

Tues 20th May

• Novagen Rosetta2 pLyS (cat# 71401)
• Promega KRX Competent cells (20 x 50 uL - cat# L3002)
• Fisher 15ml tubes
• Fisher 50ml tubes
• Lab coats XXL light blue x3
• 0.1ul-20ul tips in box x3
• 100ul-1000ul tips in box x4
• 5ml tips in box x2
• 2ul-200ul tips in box x4

13 May

• Ammonium Sulphate 2x500g
• IPTG 2x10g
• 4L flask x8
• Face Shield sign x2
• Face Shield x2
• XL lab coat x6
• XXL lab coat x3

• HiTrap Desalting column 5 × 5 ml 17-1408-01 £112.00 GE life sciences
• dodecyl maltoside 5g

arrived (ordered 1st May 2008)

• BPE1422-2 Yeast Extract 2kg x1
• BPE-310-500 HEPES Crystals 500g x1
• BPE328-1 Sodium Hydrogen Carbonate x1
• CRY-190-010Q Cryobox 10pk x2
• FB50323 Weighing boat 5ml 1000pk x1
• FB50325 Weighing boat 30ml 1000pk x1
• FB50327 Weighing boat 100ml 1000pk x1
• FB50377 Weighing boat 250ml 1000pk x1
• CRY-595-030P Centrifuge tubes 35ml 10pk x2

arrived 22nd May 2008

• 50 ml Falcon Tubes
• 15 ml Falcon Tubes
• Petri Dish Rack

APRIL

ordered 11th April 2008 Fisher order 7515525 1104081

• 10pk 500ml beakers
• 2 x 5pk 1L beakers
• 10pk 250ml beakers ordered but out of stock til May!
• 10pk 100ml glass beaker
• 10pk 250ml glass beaker
• 10pk 600ml glass beaker
• 3x 1L glass beaker
• 2 x 1000pk PCR tubes 0.2ml
• 1.5ml tube float rack 4pk
• 0.5ml tube float rack 4pk
• 25g low melting agarose
• 5 thermometers -10 to 110 degrees
• 0.5ul-10ul tips 10 x 1000pk ordered but out of stock til May!

ordered 7th April 2008 Screw Fix

• 3 shelf units for cold room

ordered 2nd April 2008

• Fisher Incubator Shelf & 2 Supports x2 ING-205-502L

Week 2

11th April 2008

• ELGA cartridges LC148, LC163, LC181, CT all x2

10th April 2008

• Steps/ladders from screw fix

9th April 2008

• Fisher 100 mg anhydrotetracycline (not Louise's GPC)

8th April 2008

Screw Fix

• Trolley for lab move and/or fermentor
• Spirit level for lab
• 6 containers for transporting bottles across lab
• Mobile step for office

Week1

3rd April 2008

• Fisher 5L Beakers x5 FB33119 £11.74 each
• Fisher Magnetic Retriever x1 FB55669
• Fisher Stirrer fleas 10pk x1 FB55601
• Fisher Stirrer fleas 10pk x1 FB55591

MARCH

31th March 2008

ordered 26/03/08

• BPE2543-2 Thrombin, bovine plasma Fisher BioReagents from Fisher Scientific
• Merck- n-Dodecyl-b-D-maltoside, ULTROL® Grade Cat. No. 324355 5g

28th March 2008

ordered 27/03/08

• Fisher- Protein Concentrators 2ml 10KMW Millipore
• Fisher- Containers for agar plates

27th March 2008

• Ultra violet DNA gel lamp and spares
• DNA marker (e.g. Fisher BPE2553-100)
• GE Healthcare XK 16/20 column 18-8773-01
• GE Healthcare HiPrep 16/10 DEAE-FF 17-5090-01

20th March 2008

• ordered 07/03/2008
• 3kg balance - Fisher
• 0.5ul-10ul tips 1000pk - Fisher

13th March

• ordered 12/03/2008
• T4 Polynucleotide kinase - recombinant from E.Coli Fisher BioReagents 100 U BPE3212-1 £23.50 Fisher Scientific
• PCR tube Fisherbrand thin walled domed cap polypropylene 0.2mL natural pack of 1000 FB68720 £64.60 Fisher Scientific x2

These inc VAT £131.55 Ref.7482996 Gail x2234

• ordered 11/03/2008
• Fisher GHL-415-010D gas burner lighter x2
• Fisher FB67020 pH Electrode x1
• Fisher FB70260 Timer x6
• Fisher BMD-110-021W Batteries x6
• Fisher LAB-550-010W white tape x1
• Fisher LAB-551-010Q Orange tape x1
• Fisher LAB-553-010E Red tape x1
• Fisher LAB-555-010E Yellow tape x1
• Fisher LAB-557-010D Green tape x1
• Fisher LAB-559-010X Blue tape x1
• Fisher LAC-730-010K Black pen 10pk x1
• Fisher LAC-730-020H Blue pen 10pk x1
• Fisher LAB-340-030Q Round labels 1000pk x1
• Fisher HW1-LB-S small lab coat x9
• Fisher HW1-LB-M Medium lab coat x9
• Fisher FB55301 5pk Clamp set
• Fisher FB68403 Base for clamp x2
• Fisher FB55305 Bosshead Clamping screws 5pk x1
• Fisher FB34531 1-200ul tips 1000pk x10
• Fisher FB34511 0.5ul-10ul tips 1000pk x10
• Fisher FB34611 200-1000ul tips 1000pk x10
• Fisher FB70173 cleaning detergent
• Total of order inc VAT £1367.22

12th March

• Sigma low range molecular weight markers Sigma 6.5 - 66 kDa M3913-10vl x1
• Sigma Mid range molecular weight markers Sigma 6.5 - 200 kDa S8445-1vl x4
• Sigma high range molecular weight markers Sigma 36 - 20 kDa S8320-10vl x1
• Sample buffer solution Sigma S3401-10vl x2
• LDao (n,n-Dimethyldodecylamine n-oxide) Sigma D9775-SG x1
• Spatulas 0.09inch tip Sigma Z243213-2EA £11.80 x10

11 March

• ordered 10/03/2008
• Promega Wizard Gel and PCR Clean up Kit 250rx A9282 x1
• GEHealthcare - PEEK tubing 1/16", i.d. 0.75 mm 2 M 18-1112-53 1 £50.50 £50.50
• Superloop, 1/16" fittings (ÄKTAdesign) 10 ml 1 EA 18-1113-81 1 £360.00 £360.00 Sub Total: £410.50
• n-dodecyl beta-D-maltose D4641-SG - Merck
• cAMP-Merck
• BL21 (DE3) competent cells -Merck

10 march

• 2 x 100g electrophoresis agarose - Fisher
• 1Kg Ammonium Acetate - Fisher
• 1ul-200ul tips 1000pk - Fisher
• 250ul-1000ul tips 1000pk - Fisher
• 3.5 KDa cut off dialysis tubing
• PCR thermocycle control panel
• Miniplasmid kits
• Acetic acid
• DTT
• glycine
• sodium bicarbonate
• EDTA
• chloramphenicol
• butane cartridges for bunsen
• ethidium bromide
• kanamycin sulphate
• ampicillin
• 0.45 u sterile filters

4 march

• Top 10 cells
• Arabinose

FEBRUARY

25 Febuary=

• Yeast extract
• 0.22 um u sterile filters
• X-gal
• KCl
• KH2Phos
• Na2Hphos
• Triton X-100
• biohazard autoclave gloves
• bunsen burner
• batteries
• orange bins
• SDS

20 February

• 1000 MWCO Spectrapor dialysis tubing
• ammonium acetate
• lysozyme