ISISBio:Internal/Group meeting/20-June-08

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Visitor from Kings for next week

  • plan to transform two plasmids, this afternoon into Top10, Bl21(DE3), KRX
  • out of incubator tomorrow morning ready for overnight cultures on Monday
  • grow Tuesday and overnight for KRX
  • start purification on Wednesday
  • keep things simple for Thursday
  • gel filtration Friday


  • have two colonies - might be the right thing
  • recloning of sortase - redoing because got no colonies
  • hope to be growing up next week

Vicky G-S

  • still haven't confirmed whether made the right thing or not
  • waiting on enzyme to test that
  • amplified up all other fusion partners

Mark Telling

  • Will try to schedule GFP experiments for September or later