IGEM:metu/2009/Notebook/wound dressing/2009/10/15

From OpenWetWare
Jump to: navigation, search
Igem-logo-150px.png WOUND DRESSING Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png

Wound dressing logo.jpg


1. To obtain large amounts of protein (EGF), again we prepared LB-Broth.

250 ml LB+ Lard 1+ABC

  • This substance was put into shaker, 37 °C and 190 rpm. (Overnight incubation - 16 hours)

2. Collagen sponge wells were taken.

  • Keep the wells at -80 C at 24 hour.
  • Lyophilize the frozen solution.

3. Starting to preparation of gelatin sponge.