IGEM:metu/2009/Notebook/wound dressing/2009/10/15

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15.10.2009 1. To obtain large amounts of protein (EGF), again we prepared LB-Broth. 250 ml LB+ Lard 1+ABC This substance was put into shaker, 37 °C and 190 rpm. (Overnight incubation - 16 hours) 2. Collagen sponge wells were taken. Keep the wells at -80 C at 24 hour. Lyophilize the frozen solution. 3. Starting to preparation of gelatin sponge.