IGEM:metu/2009/Notebook/wound dressing/2009/10/12

From OpenWetWare
Jump to: navigation, search
Igem-logo-150px.png WOUND DRESSING Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png

Wound dressing logo.jpg


1. We loaded our digested products to Agarose gel. (Electrophoresis)

2. Gel Extraction of Backbone 4 and Backbone 9.

Again, the other plasmids were not digested.

3. Starting to preparation of collagen foam.

  • Solve 48,8 µg collagen in 6,5 ml 0,05 M acetic acid to prepare 0,75 % collagen solution (the final concentration of collagen solution is 7,5 mg/ml)
  • Overnight the collagen solution at 350 C with magnetic heater to dissolve the collagen in acetic acid completely.