IGEM:metu/2009/Notebook/wound dressing/2009/10/12

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12.10.2009

1. We loaded our digested products to Agarose gel. (Electrophoresis)

2. Gel Extraction of Backbone 4 and Backbone 9.

Again, the other plasmids were not digested.

3. Starting to preparation of collagen foam.

  • Solve 48,8 µg collagen in 6,5 ml 0,05 M acetic acid to prepare 0,75 % collagen solution (the final concentration of collagen solution is 7,5 mg/ml)
  • Overnight the collagen solution at 350 C with magnetic heater to dissolve the collagen in acetic acid completely.