IGEM:Yale/2010/sandbox
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| Digestion Reaction Components | ||
|---|---|---|
| phsABC Digestion | B0015 Digestion | pSB1C3 Digestion |
| 5 μL EcoRI Buffer 10x | 5 μL NEB Buffer 3 10x | 5 μL EcoRI Buffer 10x |
| 0.5 μL BSA 100x | ||
| 21.1 μL phsABC soln (1 μg DNA) | 3.8 μL B0015 soln (1 μg DNA) | 15 μL phsABC soln (375 ng DNA) |
| 1.8 μL EcoRI & 1.8 μL SpeI | 1.8 μL XbaI & 1.8 μL PstI | 1.2 μL EcoRI, 1.2 μL PstI, & 1.2 μL DpnI |
| 20.8 μL H2O | 37.1 μL H2O | 15.9 μL H2O |
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