IGEM:Yale/2010/Protocols/ethanol precipitation

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Standard DNA Precipitation Protocol

  1. Add one-tenth volume of 3 M NaOAc, ph 5.2, to the DNA solution to precipitated.
  2. Add 2.5 volumes of cold 100% ethanol.
  3. Place in -80°C freezer for 30 minutes of in an ethanol/dry ice bath for 5 minutes.
  4. Spin at 15,000 rpm in microcentrifuge for 30 minutes at 4°C.
  5. Rinse with cold 70% ethanol.
  6. Spin at 15,000 rpm in microcentrifuge for 10 minutes at 4°C.
  7. Remove supernatant, taking care of pellet, and dry fro 1 hour at 37°C.
  8. Resuspend in appropriate volume of buffer.