SpeI Activity Assay
- To determine if SpeI being used was functional, ran a gel of circular B0015 vs. B0015 digested with SpeI. Gel was 1.0% agarose and run at 90 V with a 1 kb ladder. The result confirmed the activity of SpeI.
Gel to determine SpeI activity From left, Lane 1: 1 kb ladder, Lane 3: undigested B0015 (dimer and monomer visible), Lane 5: B0015 from SpeI digest, at 3.2 kb as a linear fragment ought