IGEM:Virginia/2012/Notebook/Genetically engineered bacteriophage for diagnosis of whooping cough/2012/07/29

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(7/29 – 8/4)

We found that the colonies grew on the negative control LB/Km plate for the transformation. It could be due to contamination.
We then plated RB50 and RB53 on LB/strep/gen+ plate
Our Gibson Assembly did not work.
We ordered primers and optimized hCG through IDT
Did our PCR cleanup on PET30a to remove contaminants