IGEM:Virginia/2012/Notebook/Genetically engineered bacteriophage for diagnosis of whooping cough/2012/07/29

From OpenWetWare
Jump to: navigation, search
Igem-logo-150px.png Week 10 <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>      </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

(7/29 – 8/4)

We found that the colonies grew on the negative control LB/Km plate for the transformation. It could be due to contamination.
We then plated RB50 and RB53 on LB/strep/gen+ plate
Our Gibson Assembly did not work.
We ordered primers and optimized hCG through IDT
Did our PCR cleanup on PET30a to remove contaminants