IGEM:University of Illinois Urbana Champaign/2009/Notebook/Bioware 2010 Gold Bioremediation/2010/07/09

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July 5th to July 9th

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Amanda: Tried different PCR mixes and protocols to try to clone out GesABC and Gol genes. Digested and ligated anything and everything that appeared on our gel.

Tom: First week trying to clone golB and golS into a plasmid. Didn't work and we discovered that gel extraction/pcr purification causes us to lose almost all of our DNA.