IGEM:University of Illinois Urbana Champaign/2009/Notebook/Bioware 2010 Gold Bioremediation/2010/07/02

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June 28th to July 2nd

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Amanda: Tried PCR of GolT, B, and S, as well as GesABC. Did a lot of PCR troubleshooting.

Tom: After we gave up on PCRing gesABC and golT, I started amplifying golB and golS from the first successful PCR and began trouble shooting why we've been having problems with PCR. Also met with Dr. Rao a couple of times about primers and got protein gel stuff from Joanne.