IGEM:University of Illinois Urbana Champaign/2009/Notebook/Bioware 2010 Gold Bioremediation/2010/05/28

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Summary of Week 1: May 24th to May 28th

Tom: Research - Metal reduction and metal resistance. Practicing basic lab techniques like PCR, cloning, plating and combining biobricks. Also learned how to design primers.

Amanda: We spent a lot of time brainstorming and researching project ideas; mainly, metal resistance/precipitation and magnetosome formation. I learned a lot about metal resistance, am still not sure about precipitation. In lab we put together biobricks. This involved learning protocols for digestion, pcr cleanup, ligation, transformation, miniprep, pcr, making spreadplaters!! Also learned about primer design and searching for genes/sequences on microbesonline from dr. rao. Joanne talked to us about publicity ideas such as Surrounded by Science at parkland, GAMES, and other stuff I'm excited to be a part of! The barbecue was a lot of fun and I got 2 mosquito bites on my forehead and two on my arm.