IGEM:University of Illinois Urbana Champaign/2009/Notebook/Bioware 2010 Arsenic Bioremediation/2010/08/16

UIUC - Arsenic Collection System!

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8/16/2010 3:00PM PCR Ingredient Amount Mastermix (4 reactions) 10X Buffer 2 μL 8 μL 10 mM dNTPs .4 μL 1.6 μL Template 1 μL (18 ng) added individually F Primer .4 μL 1.6 uL R Primer .4 μL 1.6 uL DMSO 1 μL/td> 4 μL Pfu Polymerase .3 μL 1.2 μL H2O 14.5 μL 58 μL Per Reaction: 19 μL Mastermix, 1 μL Template. Samples: LamB EcoRI LamB EcoRI Negative control (no template) Primers: LamB F2 EcoRI LamB R Program: 95°C 3 min 95°C 30 sec 54°C 30 sec 72°C 2 min go to step 2 33X 72°C 5 min hold at 4°C 4:50PM 1st Streak of ISB207 - ArsR SDM2 pSB1A2 on Amp