IGEM:University of Illinois Urbana Champaign/2009/Notebook/Bioware 2010 Arsenic Bioremediation/2010/08/16

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8/16/2010

3:00PM

PCR

Ingredient Amount Mastermix (4 reactions)
10X Buffer 2 μL 8 μL
10 mM dNTPs .4 μL 1.6 μL
Template 1 μL (18 ng) added individually
F Primer .4 μL 1.6 uL
R Primer .4 μL 1.6 uL
DMSO 1 μL/td> 4 μL
Pfu Polymerase .3 μL 1.2 μL
H2O 14.5 μL 58 μL

Per Reaction: 19 μL Mastermix, 1 μL Template.

Samples:

  • LamB EcoRI
  • LamB EcoRI
  • Negative control (no template)

Primers:

  • LamB F2 EcoRI
  • LamB R

Program:

95°C 3 min
95°C 30 sec
54°C 30 sec
72°C 2 min
go to step 2 33X
72°C 5 min
hold at 4°C

4:50PM 1st Streak of ISB207 - ArsR SDM2 pSB1A2 on Amp