IGEM:University of Illinois Urbana Champaign/2009/Notebook/Bioware 2010 Arsenic Bioremediation/2010/07/09
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UIUC - Arsenic Collection System! | Main project page Previous entry Next entry | |||||||||||||||||||||||||||||||||||||
7/9/10Colony Boil 50 ul H2O, 3 colonies. gave 25 ng/ul DNA. PCR
Per Reaction: 19.2 ul mastermix, .4 ul F Primer, .4 ul R primer Primers pArsR F1 pArsR F2 pArsR R1 pArsR R2 ArsR F RFC10 EcoRI ArsR R RFC10 ArsR R fusion BSAI ArsR F Fusion BSAI LamB F1 RFC10 XbaI LamB F2 RFC10 XbaI LamB R RFC10 SpeI Samples LamBF1R LamBF2R ArsR Fusion ArsR pArsR F1R1 pArsR F1R2 pArsR F2R1 pArsR F2R2 Negative control (no primers) Program
Meagan did transformation of yesterday's ligation at 3:55 PM, incubated for an hour at 37°C and plated 200 ul on Tet plate at 4:55 PM. |