IGEM:University of Illinois Urbana Champaign/2009/Notebook/Bioware 2010 Arsenic Bioremediation/2010/07/06
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At this point, we ran a gradient PCR of Pal and one ArsR sample. It didn't work at all - no bands on the gel, except the ladder. The extension time was 1 minute with 30 cycles. 95 - 2min (95 - 30sec; 55ish - 30sec; 72 - 1min | 30x) 72 - 1 min 4 - hold
Run at 100V for 30min - No Bands (except the ladder)
Gel of Digests
Run at 90V
Each reaction gets 32 ul mastermix, 4 ul of each primer.
ArsR R RFC10
ArsR F RFC10 EcoRI
7. negative control, no primers
Band extraction of pSB1A2 from gel
re-streaked e. Coli from yesterday for 2nd overnight for colony boils.
Put transformations from 7/6 12am - Erin into 4C room - No Colonies
Everyday is a Monday in the life of Erin
Hi. My name is Erin. I am also referred to as, "The Bork", "Borky" and "PB & J". Although I never warmed to these terms of affection, I understand that they do reflect my persona and thus my essence, very well. I am very tired.
It is during this time, when I'm sleep deprived, overworked, sore, and stressed, when I begin to lose myself in various philosophical quandaries. Today's topic of interest does not lie in the archaic ramblings of Plato or Aristotle, but rather in the quarky, uncanny ability of Steve's hairline to recede to his occiput. How such a phenomenon occurs has reduced my intellectual capacitance to mere shapes: squares, circles, you get the point. Every day, it's like watching the waves rise of the shore, leaving a residual bump, scarcely noticeable, save for the keen of eye. But he's begun to grow his hair out, which has worked only to thwart my amusement.
I'll wait till he gets a haircut.