IGEM:University of Illinois Urbana Champaign/2009/Notebook/Bioware 2010 Arsenic Bioremediation/2010/07/01
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Meagan plated the cells transformed last night at 12:30 AM. We observed no growth on the plates this morning when we checked at 9:30 AM for both those plates and the PAL_pSB1A2 plate.
Ran ligations and digests on gels at 80V.
incubate at room temperature for 10 minutes
inactivate at 80°C for 20 minutes
100 ul cells + 5 ul ligation product (J23100-Gas Vesicles-pSB1T3 or J23119-Gas Vesicles-pSB1T3). electroporate and add 1 mL LB, incubate at 37°C for 1 hour for recovery.
Plated 20ul transformation product on 1/2 tetracycline plate, plated 200 ul on other half. Next day observed no growth.