IGEM:University of Illinois Urbana Champaign/2009/Notebook/Bioware 2010 Arsenic Bioremediation/2010/05/25

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5/25/10 Bootcamp Day 2 Digestion/Ligation Amount for 500ng DNA 120 4.26 ul 12M 2.69 ul J23100 2.17 ul (promoter) BBa_J04450 1.76 ul (backbone) Post-Ligation Concentrations J23100-12M-BBa_J04450 312.6 ng/ul J23100-12O-BBa_J04450 302.13 ng/ul Transformations/Plating For both 12O and 12M constructs: 50 ul transformed cells on chloramphenicol plate 100 ul transformed cells on chloramphenicol plate