IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/09/23

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Floor Two

The plates and cultures from the previous day were retrieved. Colonies had grown on the plates, but neither them or the cultures had a red colour as aspected. 5ml from each culture was used to purify a soluble protein sample and the remaining 5ml from each was used for minipreps to retrieve the DNA. All samples were put into the -20°C freezer overnight.
The sequencing reaction for 3a appeared to be unsuccessful.