IGEM:University of Chicago/2009/Notebook/PNP Biosensor/2009/08/13

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August 13th, 2009

measured DNA conc. using nanodrop appartus. this DNA was cut using PacI and AscI (overnight) and then gel purified.

Ligation (super GFP into Longtine Plasmids [Kan and His])

20 ul RXNs

4 5 x buffer

2. plasmid

2. insert

1 igase

11 ddH2O

Ran four reactions

1. S-GFP + His Plasmid

2. S-GRFP + kan plasmid