IGEM:UNAM-Genomics Mexico/2009/Notebook/H2 Pae/2011/08/04

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Objectives

  • To continue the conjugation

Continuing conjugation

   * I took all the bacteria which grown overnigth and I put them into a test tube containing 1mL of MgSO4 and mixed with vortex.
   * I took 100 μL of the previous test and passed them to a test tube containing 900μL of MgSO4.(First dilution). I repeted this until reached third dilution.
   * I plaqued 100 μL of the second an thir dilution in a selective Py medium, contaning Nal an Gm because R. etli is natural resitant to Nal and if R. etli is correctly cojugated will aquire Gm resistance through pBBR1MCS-5.
   * The cultives will be left until tuesday