IGEM:UC Berkeley/2006/Short DNA frag cleanup

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  • Use this procedure for cleaning up digestions, PCRs, Extensions, etc. on short fragments of DNA ~<300bp
  1. Add one volume of ADB Buffer to your solution.
  2. Add 600uL EtOH
  3. Spin resulting solution through a ZYMO column
  4. Wash two times with 200uL of PE buffer
  5. Spin dry for like 2 minutes
  6. Elute with water