IGEM:UC Berkeley/2006/KCMSmallScale
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- Grow cells in ~4 mL LB until cloudy
- Put on ice
- Transfer 1mL into an eppendorf tube on ice, let cool
- Centrifuge full speed for 30 sec, toss out supernatant
- Resuspend in 90uL of TSS solution
- Add 10uL KCM
- Add 1uL plasmid DNA
- Let sit on ice for 10min, heat shock 90 sec at 42, incubate and/or plate