IGEM:UC Berkeley/2006/Digestion
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Digestion of a Mini-prep DNA (35 ul scale)
- 13 uL water
- 3.5 uL buff(3)
- 3.5 uL BSA
- 1 uL Enzyme 1
- 1 uL Enzyme 2
- 15 uL DNA
35
INCUBATE AT 37 degrees for 2 hours!
Digestion of a Mini-prep DNA (20 uL scale)
- 10 uL DNA
- 7 uL water
- 2 uL 10x buffer
- 0.5 uL Enzyme 1
- 0.5 uL Enzyme 2
20
Scaled up digestion of mini-prep
- 14uL H2O
- 4uL NEB Buffer 2
- 20uL DNA
- 1uL restriction enzyme
- 1uL restriction enzyme
40
Digestion of PCR Product
- Elute 108uL of water
- 12 uL of 10X NEB Buffer 2
- 1 uL Enzyme 1
- 1 uL Enzyme 2
120 uL