IGEM:UC Berkeley/2006/Analitic digestion

1. Use Ape to determine which enzymes to cut product with. Cuts should yield bands that are easily distinguishable on a gel.
2. To digest make a cocktail of NEB2, Enzyme, H2O, and DNA product for a total volume of 10uL.
   • If DNA product is high copy then use only 2uL of product 1uL of NEB2 and adjust the H2O and enzyme accordingly to make 10uL total:
     • • 2 uL miniprep/DNA product
       • 1 uL NEB2
       • .25 uL enzyme 1
       • .25 uL enzyme 2
       • 1 uL BSA
       • 5.5 uL water
   • If DNA product is low copy then use 4uL of product and 1uL of NEB2 adjusting the H2O and enzyme accordingly:
     • • 4 uL miniprep/DNA product
       • 1 uL NEB2
       • .25 uL enzyme 1
       • .25 uL enzyme 2
       • 4.5 uL water
3. Incubate for ~30min. and then run on gel.