Biofilm Track
Eric F. + Melody
Biofilm (100816M + 100816E) Growth Protocol
- Using Melody's 3mL cultures in plastic tubes of RN4220 + 8325-4
- Inoculated flat bottomed 96 well plate with 1% glucose TSB RN42220 and 8325-4 in the below arrangement
- Both plates were done exactly the same, at different times
Biofilms 100816M + 100816E Arrangement
| Columns |
| Rows |
|
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
| A |
X |
X |
X |
X |
X |
X |
X |
X |
X |
X |
X |
X |
| B |
X |
X |
X |
X |
X |
X |
X |
X |
X |
X |
X |
X |
| C |
X |
X |
C |
R |
R |
R |
8 |
8 |
8 |
C |
X |
X |
| D |
X |
X |
8 |
8 |
8 |
C |
C |
R |
R |
R |
X |
X |
| E |
X |
X |
C |
R |
R |
R |
8 |
8 |
8 |
C |
X |
X |
| F |
X |
X |
X |
X |
X |
X |
X |
X |
X |
X |
X |
X |
| G |
X |
X |
X |
X |
X |
X |
X |
X |
X |
X |
X |
X |
| H |
X |
X |
X |
X |
X |
X |
X |
X |
X |
X |
X |
X |
- I think Will check with the lab book tomorrow
- Note: C = control, R = 1% glucose RN4220, 8 = 1% glucose 8325-4
- 100816M (Melody's plate) went in at 1722
- 100816E (Eric's plate) went in at 1830
dspB Track
O/N culture for miniprep
- 3T1,3T2,4T1,4T2 in shaker @ 37C @1632
QS Track
- Noticed agrCA from distribution came from wrong kit plate (it is on plate 2 instead of 1).
- Resuspended agrCA in 15 μL dH2O and then transformed into DH5alpha cells using UBC iGEM protocols.
- Digested and Ligated agrCA (natural Staph form) with B0014 (terminator) onto a PsB1C3 backbone. --Followed Biobrick protocols
- Digestion time: 30 mins. Ligation Time: 50 mins.
- agrCA was not purified using a spin column
- Transform ligation mixture following UBC iGEM protocol. Use all 20 μL.
|
iGEM Project name 1
Main project page
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