IGEM:The University of Sheffield/2009/Notebook/Sheffield iGEM 2010/2010/09/22

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Wednesday 22nd September

  • The re-streak of the co-transformation grew on the Amp, Cm agar plates.
  • Carried out plasmid mini preps of the E.coli containing the Chimeric sequence and of E.coli containing the HapR promoter. [[1]]
  • Overnight restriction digests were set up using the two mini preps of the chimeric sequence and the hapR promoter:
 Plasmid - 10μL
 Pst1 - 2μL
 EcoR1 - 2μL
 EcoR1 buffer - 2μL
 BSA (x20) - 1μL
 H2O - 3μL