IGEM:The University of Sheffield/2009/Notebook/Sheffield iGEM 2010/2010/09/22

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Wednesday 22nd September The re-streak of the co-transformation grew on the Amp, Cm agar plates. Carried out plasmid mini preps of the E.coli containing the Chimeric sequence and of E.coli containing the HapR promoter. [[1]] Overnight restriction digests were set up using the two mini preps of the chimeric sequence and the hapR promoter: Plasmid - 10μL Pst1 - 2μL EcoR1 - 2μL EcoR1 buffer - 2μL BSA (x20) - 1μL H2O - 3μL