IGEM:The University of Sheffield/2009/Notebook/Sheffield iGEM 2010/2010/08/16

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Monday 16th August:

  • Poured more antibiotic agar plates, (Km, Amp, Km + Amp)
  • Set up a restriction digest with pACYC, pCDF and pCOLA with EcoR1 and Pst1 enzymes:
  Plasmid- 10μL
  Pst1 - 2μL
  NcoR1 - 2μL
  EcoR1 buffer - 2μL
  BSA (x20) - 1μL
  H2O - 3μL

  • Carried out gel electrophoresis [[1]] of the digested plasmids.
  • Made overnight starter cultures of MG1655 WT.
  • Made new LB.