IGEM:The University of Sheffield/2009/Notebook/Sheffield iGEM 2010/2010/07/13

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Tuesday 13th July: Practice Transformations and Competency Experiments

  • Both strains of E.coli were made chemically competent[[1]] using the overnight cultures made on the 12th.
  • The now competent cells were transformed with part BBa_J45014 from the iGEM kit plates(Banana odour).[[2]] This was done to test both our transformation and competency methods.
  • After transformation the cells now containing part BBa_J45014 were spread onto ampicillin agar plates and put into a 37°C incubator overnight.