IGEM:The Citadel/Notebook/Notes Brian/06-24-10

• (12:30pm) Plated 100μL of cells containing the following parts at the specified concentrations on LB-Miller plates w/ Amp antibiotic:

Original cell solutions diluted w/ LB Lennox broth instead of LB Miller broth (Concentration values listed are relative to the original cell solution concentration) (All plates placed in 37°C incubator at 2:13pm.)

• • BBa_R0062 [50%], [25%]
  • BBa_E0240 [50%], [25%]
  • BBa_R0063 [50%], [25%]
  • BBa_B0015 [50%], [25%]
  • BBa_R0040 [50%], [25%]
  • BBa_J23102 [50%], [25%]
  • BBa_C0160 [50%], [25%]
  • BBa_J45200 [50%], [25%]
  • BBa_F2620 [50%], [25%]
  • BBa_F1610 [100%], [50%], [25%]
  • BBa_I0462 [100%], [50%], [25%]
  • BBa_J45120 [100%], [50%], [25%]
• Made 22 LB-Miller plates w/ 50μg/mL Kan antibiotic
• (3:37pm) Inoculated 10 5mL overnight cultures with the following part-laden E.coli:
  • BBa_J23102
  • BBa_R0062
  • BBa_F2620
  • BBa_R0040
  • BBa_R0063
  • BBa_B0015
  • BBa_C0160
  • BBa_J45200 (we are not 100% confident that this colony was introduced to the correct part. Confirm sequence soon.)
  • BBa_E0240
  • BBa_F1610
• Plated
  • BBa_J23102
  • BBa_R0062
  • BBa_F2620
  • BBa_R0040
  • BBa_R0063
  • BBa_B0015
  • BBa_C0160
  • BBa_J45200
  • BBa_E0240
  • BBa_F1610