IGEM:Stanford/2010/Project/Research and Development

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Research and Development

Papers of Interest

Ratiometric Sensing

Linearly Inducible Promoters

  1. Lee SK and Keasling JD. A propionate-inducible expression system for enteric bacteria. Appl Environ Microbiol. 2005 Nov;71(11):6856-62. DOI:10.1128/AEM.71.11.6856-6862.2005 | PubMed ID:16269719 | HubMed [lee]

  1. Khlebnikov A, Risa O, Skaug T, Carrier TA, and Keasling JD. Regulatable arabinose-inducible gene expression system with consistent control in all cells of a culture. J Bacteriol. 2000 Dec;182(24):7029-34. PubMed ID:11092865 | HubMed [khlebnikov]

  1. A and Keasling JD. Effect of lacY Expression on Homogeneity of Induction from the Ptac and Ptrc Promoters by Natural and Synthetic Inducers. Biotechnol Prog 2002 April; 18 672-674. [1] [Khlebnikov]

  1. Khlebnikov A, Skaug T, and Keasling JD. Modulation of gene expression from the arabinose-inducible araBAD promoter. J Ind Microbiol Biotechnol. 2002 Jul;29(1):34-7. DOI:10.1038/sj.jim.7000259 | PubMed ID:12080425 | HubMed [araBAD]
  • Figure 2 shows output as a response to arabinose concentration. The squares (filled and open) and the open diamonds seem to show a linear response curve over ~4 orders of magnitude.