IGEM:Stanford/2010/Notebook/22 April 2010

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April 22 2010

Molecular breeding of carotenoid biosynthetic pathways by Schmidt-Dannert et al.

  1. Heim R, Prasher DC, and Tsien RY. Wavelength mutations and posttranslational autoxidation of green fluorescent protein. Proc Natl Acad Sci U S A. 1994 Dec 20;91(26):12501-4. PubMed ID:7809066 | HubMed [Tsien]

Presentation by Greg

Learned about targeted evolution, family planning, and molecular breeding in the context of the carotenoid biosynthetic pathway. Tools such as these can be use to create a mutant protein of choice without undergoing the rigor of silent mutations.